| HLS | Hue-Lightness-Saturation | 色调亮度饱和度 |
| HLS | Healthcare Linen Specialists | 医疗保健亚麻专家 |
| HLS | hypertonic lactated saline solution | 高渗乳酸盐水 |
| HLS | HopeLess Score | 无希望得分 |
| HLS | His LordShip | 上帝的船 |
| HLS | High Level Synthesis | 高级合成 |
| HLS | Hook, Line, and Sinker | 吊钩、绳索和水槽 |
| HLS | Hot Looks School | Hot Looks学校 |
| HLS | St. Helens, Tasmania, Australia | 澳大利亚塔斯马尼亚州圣海伦 |
| HLS | Household Livelihood Security | 家庭生计保障 |
| HLS | High Line Speed | 高速生产线 |
| HLS | Halt Line State (Cisco) | 停止线路状态（Cisco） |
| HLS | Huntington Locomotive Shop | 亨廷顿机车车间 |
| HLS | High Level Sports | 高水平运动 |
| HLS | Hue, Luminance, Saturation | 色调、亮度、饱和度 |
| HLS | Huntingdon Life Sciences | 杭廷顿生命科学研究中心 |
| HLS | Hospital Libraries Section | 医院图书馆科 |
| HLS | Harvard Law School | 哈佛法学院 |
| HLS | Hyperbaric Lighting Set | 高压照明设备 |
| HLS | Hills | 丘陵 |
| HLS | High-Level Synthesis | 高级合成 |
| HLS | Home Library Service | 家庭图书馆服务 |
| HLS | Hard Luck Story | 不幸的故事 |
| HLS | Home Loan Servicing | 住房贷款服务 |
| HLS | Homeland Security | 国土安全部 |
| HLS | Homeland Support | 国土支持部 |
| HLS | Hot Lava Software | Hot Lava软件 |
| HLS | Higher Level Stewardship (UK; agriculture) | 高级管理（英国；农业） |
| HLS | Helicopter Landing Site | 直升机着陆场 |
| HLS | Horizontal Layered Schedule | 水平分层计划 |
| HLS | Helicopter Landing System | 直升机着陆系统 |
| HLS | Healthy Living Seminar | 健康生活研讨会 |
| HLS | Huntingdon Life Sciences (research laboratory, Cambridgeshire, England) | 亨廷顿生命科学（英国剑桥郡研究实验室） |
| HLS | Healthcare and Life Sciences | 医疗保健和生命科学 |
| HLS | Hybrid Transmission with Layered Source | 分层源混合传输 |
| HLS | Hue, Lightness, Saturation (color model, aka HVS) | 色调、亮度、饱和度（颜色模型，又名HVS） |
| HLS | Health Level Seven (healthcare) | 健康七级（医疗保健） |