| AFL | American Federation of Labor | 美国劳工联合会 |
| AFL | Actual Flight Level | 实际飞行高度 |
| AFL | Abstract Family of Languages | 抽象语言族 |
| AFL | Aside Fade Listen | 衰减后监听 |
| AFL | Association For Leather | 皮革协会 |
| AFL | Lotus 1-2-3 Font file (for Always) | Lotus 1-2-3字体文件（始终） |
| AFL | Amateur Football League | 业余足球联赛 |
| AFL | Alta Floresta, MT, Brazil | Alta Floresta，MT，巴西 |
| AFL | AFLAC, Inc. | AFLAC公司。 |
| AFL | Australia Football League | 澳大利亚足球联赛 |
| AFL | Aluminium Fluoride Litate | 硅酸氟化铝 |
| AFL | A Free Lunch | 免费午餐 |
| AFL | The Anime Fantasy League | 动漫幻想联盟 |
| AFL | Applied Fisheries Laboratory | 应用渔业实验室 |
| AFL | AbsoFreakinLutely | AbsoFreakin缓慢 |
| AFL | Autofocus Lock (cameras) | 自动对焦锁定（相机） |
| AFL | Artists for Literacy (San Francisco, CA) | 识字艺术家（加利福尼亚州旧金山） |
| AFL | Authorized Funding Level | 授权资金水平 |
| AFL | Approximate Full Load | 近似满载 |
| AFL | Aeroflot (Russian Airline, ICAO code) | 俄罗斯航空公司（俄罗斯航空公司，国际民航组织代码） |
| AFL | Auzzie Footy Legends | Auzzie Footy传奇 |
| AFL | Artificial limb | 假肢 |
| AFL | Absolute Fluid Loss | 绝对流体损失 |
| AFL | antifibrinolysin | 抗纤溶蛋白 |
| AFL | Alt Fan Letterman | Alt Fan Letterman（Alt Fan莱特曼） |
| AFL | aflatoxicol | 黄曲霉毒素 |
| AFL | The Australian Faggots League | 澳大利亚Faggots联盟 |
| AFL | anti-fatty liver | 抗脂肪肝 |
| AFL | aspergillus flavus | 黄曲霉 |
| AFL | American Football League | 美国足球联盟 |
| AFL | Air Field Lighting | 空气场照明 |
| AFL | RubberFlex animation (AnimaFlex) | RubberFlex动画（AnimalFlex） |
| AFL | Away From Link | 远离链接 |
| AFL | Australian Football League | 澳大利亚足球联赛 |
| AFL | Alberta Federation of Labour | 阿尔伯塔省劳工联合会 |
| AFL | Afloat | 阿弗洛特 |
| AFL | After Fader Listening (audio mixing) | 音量调节器收听后（音频混音） |
| AFL | Auxiliary Fuel Line | 辅助燃油管路 |
| AFL | Active Fuel Length | 有效燃油长度 |
| AFL | American Football League (1960s) | 美国足球联盟（1960年代） |
| AFL | Above Floor Level | 高于地面标高 |
| AFL | Alcoholics for Life | 终生酗酒 |
| AFL | Autoland Flight Tests | 自动着陆飞行测试 |
| AFL | Airborne Flying Laboratory | 机载飞行实验室 |
| AFL | Arena Football League | 竞技场橄榄球联盟 |
| AFL | Air Force Letter | 空军信函 |