| TSO | total system cost | 系统总成本 |
| TSO | Technical Services Operations | 技术服务运营 |
| TSO | Texas State Optical (Houston, Texas) | 德克萨斯州光学（德克萨斯州休斯顿） |
| TSO | Temporary Slow Order (railway) | 临时慢行令（铁路） |
| TSO | The Speakeasy Offensive (Counter-Strke team) | Speakeasy攻势（反击队） |
| TSO | Technology Spillovers | 技术溢出 |
| TSO | Technical Standard Order | 技术标准订单 |
| TSO | Tactical Support Operations | 战术支援作战 |
| TSO | Telecommunications Service Order | 电讯服务令 |
| TSO | Time Sharing Option | 时间共享选项 |
| TSO | Technical Service Order | 技术服务订单 |
| TSO | Technical Support Office | 技术支持办公室 |
| TSO | Tactical Systems Officer | 战术系统官 |
| TSO | Technical Staff Officer | 技术参谋 |
| TSO | Total Shares Outstanding | 已发行股份总数 |
| TSO | Tasmanian Symphony Orchestra | 塔斯马尼亚交响乐团 |
| TSO | Time Since Overhaul | 大修后的时间 |
| TSO | Transportation Security Officer | 运输安全员 |
| TSO | Telephone Service Observation | 电话服务观察 |
| TSO | Trial Services Office (US military) | 审判服务办公室（美军） |
| TSO | Trans-Siberian Orchestra | 横贯西伯利亚乐团 |
| TSO | Team Support Officer | 团队支持官 |
| TSO | Thru-Short-Open | 直通短路开路 |
| TSO | Town Sub-Office | 镇办事处 |
| TSO | Total Store Ordering (SPARC architecture consistency model) | 总体商店订购（SPARC体系结构一致性模型） |
| TSO | Transportation Supply Officer | 运输供应官 |
| TSO | Terminal Security Officer | 航站楼安全员 |
| TSO | TRICARE Support Office | TRICARE支持办公室 |
| TSO | The Sims Online | 模拟人生在线 |
| TSO | time-sharing option | 分时期权 |
| TSO | The Seraphim Order | 塞拉菲秩序 |
| TSO | Tesoro Petroleum Corporation | 特索罗石油公司 |
| TSO | traction-screw osteosynthesis | 牵引螺钉接骨术 |
| TSO | Tresco, Isles of Scilly | 特里斯科，西里群岛 |
| TSO | Technical Standards Order | 技术标准订单 |
| TSO | Type Standardization Orders | 类型标准化订单 |
| TSO | Transmission System Operator | 传输系统操作员 |
| TSO | Taxpayer Service Office | 纳税人服务办公室 |
| TSO | Time Spent Online | 在线花费的时间 |
| TSO | Top Secret Organization | 绝密组织 |
| TSO | Terminal System Operation | 终端系统操作 |
| TSO | Tsonga | 聪加人 |
| TSO | Temporary Standing Order (US DoD) | 临时常规命令（美国国防部） |
| TSO | Tactical Sensor Operator (military aviation) | 战术传感器操作员（军用航空） |
| TSO | Test Support Office | 测试支持办公室 |
| TSO | The Special One (nickname of Jose Mourinho) | 特别一人（穆里尼奥的绰号） |
| TSO | Technisch Secundair Onderwijs (Dutch) | Technisch Securair Onderwijs（荷兰语） |
| TSO | Trans-Siberian Orchestra (band) | 跨西伯利亚乐团（乐队） |
| TSO | Transmission System Operator (energy) | 输电系统操作员（能源） |
| TSO | Timberline Symphony Orchestra (Lafayette, CO) | 林柏林交响乐团（科罗拉多州拉斐特） |
| TSO | Toronto Symphony Orchestra (Canada) | 多伦多交响乐团（加拿大） |
| TSO | Trading Standards Officer | 交易标准官员 |
| TSO | Trichuris Suis Ova (pig whipworm eggs) | 猪鞭虫卵 |
| TSO | The Sims Online (gaming) | 模拟市民在线（游戏） |
| TSO | The Stationery Office (UK) | 文具办公室（英国） |
| TSO | Transaction Server Operation | 事务服务器操作 |
| TSO | Tempe Symphony Orchestra (Tempe, AZ) | 坦佩交响乐团（亚利桑那州坦佩） |
| TSO | Time Stamp Ordered | 订购的时间戳 |
| TSO | Transmission Systems Operations/Operator (telecommunications) | 传动系统运作及操作(电信) |
| TSO | Telecommunications Service Obligations (New Zealand) | 电信服务义务（新西兰） |
| TSO | Tight Shut Off (emergency system valve feature) | 紧密关闭（紧急系统阀功能） |
| TSO | Teamspeak Overlay (gaming) | Teamspeak Overlay（游戏） |