| RLS | Renaissance Lawyer Society | 复兴律师协会 |
| RLS | Road Locomotive Society (UK) | 道路机车协会（英国） |
| RLS | Real Life Scenario | 真实生活场景 |
| RLS | Row Level Security | 行级别安全性 |
| RLS | rat lung strip | 大鼠肺条 |
| RLS | Root Lateral Spread | 根部横向扩展 |
| RLS | Roccus Leneatus Saxatilis | 虎尾大眼岩（Rocus Leneatus Saxatilis） |
| RLS | ringer lactate solution | 林格乳酸溶液 |
| RLS | person who stammers having difficulty in enuncating r,l and s | 结结巴巴难以辨认r、l和s的人 |
| RLS | Rainbow Lake Software | 彩虹湖软件 |
| RLS | River of Life Sanctuary | 生命之河保护区 |
| RLS | Regional Library System | 区域图书馆系统 |
| RLS | Retirement Living Service | 退休生活服务 |
| RLS | Retirement Living Services | 退休生活服务 |
| RLS | Ricks Loyal Supporters | Ricks忠实支持者 |
| RLS | Ralph Lauren Sports | 拉尔夫·劳伦体育 |
| RLS | R L Studebaker | R L Studebaker公司 |
| RLS | Restless Legs Syndrome | 不安腿综合征 |
| RLS | Restless Leg Syndrome | 不安腿综合征 |
| RLS | Rate Limiting Step | 速率限制步骤 |
| RLS | Residential Living Supervisor | 住宅生活主管 |
| RLS | Rail Load Simulator | 轨道荷载模拟器 |
| RLS | Return to Launch Site (also seen as RTLS) | 返回发射场（也称为RTLS） |
| RLS | Residual Life of the System | 系统的剩余寿命 |
| RLS | Radar Line of Sight | 雷达视线 |
| RLS | Robert Louis Stevenson | 罗伯特·路易斯·史蒂文森 |
| RLS | Registered Land Surveyor | 注册土地测量师 |
| RLS | Replica Location Service | 副本位置服务 |
| RLS | Received Line Signal | 接收到的线路信号 |
| RLS | Real Life Soap (Wiki) | 真实生活肥皂（Wiki） |
| RLS | Residence Life Staff | 居住生活人员 |
| RLS | Recursive Least Squares (a mathematical algorithm) | 递归最小二乘法（一种数学算法） |
| RLS | Resource Loaded Schedule | 资源加载计划 |
| RLS | Resource List Server | 资源列表服务器 |