| TLS | Transport Layer Security | 传输层安全 |
| TLS | Toque Limiting System | 转矩限制系统 |
| TLS | Toulouse, France - Blagnac (Airport Code) | 法国图卢兹-布拉尼亚克（机场代码） |
| TLS | Tunable Laser Source | 可调谐激光光源 |
| TLS | Transparent LAN Service | 透明LAN服务 |
| TLS | Terminology and Lexical Semantics (International Workshop) | 术语和词汇语义学（国际研讨会） |
| TLS | Target LAN Server | 目标LAN服务器 |
| TLS | Times Literary Supplement | 《泰晤士报》文学副刊 |
| TLS | Text Library System (Financial Planning Consultants, Inc.) | 文本库系统（财务规划顾问公司） |
| TLS | Total Least Squares | 总最小二乘法 |
| TLS | Top Level School (USMC) | 顶级学校（USMC） |
| TLS | Transaction Layer Security | 事务层安全 |
| TLS | Timor Leste (East Timor ISO code) | 东帝汶（东帝汶ISO代码） |
| TLS | Thread Local Storage | 线程本地存储 |
| TLS | Tumor Lysis Syndrome | 肿瘤溶解综合征 |
| TLS | Thesaurus & Linguistic Integrated System (IBM) | 同义词典和语言集成系统(IBM) |
| TLS | Transponder Landing System | 应答器着陆系统 |
| TLS | Trimmed Leaf Size (printing/publishing) | 修剪叶子的大小(印刷) |
| TLS | Top Level Specification | 顶层规范 |
| TLS | Table of shared Locked States | 共享锁定状态表 |
| TLS | Time Line Sheet | 时间线表 |
| TLS | Tape Library System | 磁带库系统 |
| TLS | Telstra, LTD. | 澳大利亚电信有限公司。 |
| TLS | The Last Saiyans | 最后的塞亚人 |
| TLS | The Love Shack | 爱情小屋 |
| TLS | Truck Load Services | 卡车装载服务 |
| TLS | Tiverton Library Services | 蒂弗顿图书馆服务 |
| TLS | Together Learning and Sharing | 共同学习和分享 |
| TLS | Transmission Line Speakers | 传输线扬声器 |
| TLS | The Last Survivors | 最后的幸存者 |
| TLS | The Lost Sombrero | 迷失的索姆布雷罗 |
| TLS | Training For Learning And Serving | 学习和服务培训 |
| TLS | Torrey Life Sciences | 托瑞生命科学 |
| TLS | Transparent Lan Services | 透明局域网服务 |
| TLS | Telephone Locking System | 电话锁定系统 |
| TLS | Toulouse, France | 法国图卢兹 |
| TLS | Telephone Line Simulator | 电话线模拟器 |
| TLS | Translucent Liquid Sculpey | 半透明液体飞毛腿 |
| TLS | Turbo Lycoming Sabre (aircraft engine) | 涡轮莱康明刀（飞机发动机） |
| TLS | The Logan School (Denver, Colorado) | 洛根学校（科罗拉多州丹佛） |
| TLS | Translocated in Liposarcoma-associated protein | 脂肉瘤相关蛋白的转运 |
| TLS | Tactical Landing System | 战术着陆系统 |
| TLS | Transmit Level Sensor | 发射电平传感器 |
| TLS | Transmitter Location Systems, LLC | 发射机定位系统有限责任公司 |
| TLS | The Last Samurai (movie) | 最后的武士（电影） |
| TLS | Thermal Laser Stimulation | 热激光刺激 |
| TLS | Training-Leader Development-Soldier Support | 训练领导发展士兵支持 |
| TLS | Transport LAN Service (US West) | 传输局域网服务（美国西部） |
| TLS | test of limit for sex | 性极限试验 |
| TLS | Trans-cowl Locking System (Pratt & Whitney) | Trans-cowl锁定系统(Pratt &惠特尼) |
| TLS | The Last Stop | 最后一站 |
| TLS | Transformed Least Squares | 变换最小二乘法 |
| TLS | Two Level Systems | 两级系统 |
| TLS | Transparent Liquid Sculpey | 透明液体飞毛腿 |
| TLS | Technical Layer Security | 技术层安全 |
| TLS | Three Line Scanner | 三线扫描仪 |
| TLS | Traditional Lough Style | 传统Lough风格 |
| TLS | The Last Supper | 最后的晚餐 |
| TLS | Total Logistics Support | 总后勤支持 |
| TLS | Time-Line Summary | 时间线摘要 |
| TLS | Through Life Support | 通过生命支持 |
| TLS | Theater Logistics Station | 剧院后勤站 |
| TLS | Trinity Lutheran School | 圣三一路德学校 |
| TLS | Taiwan Light Source | 台湾光源 |
| TLS | Total Logistics Support (Navy) | 总后勤支持（海军） |
| TLS | Two-Layer Spreading | 双层摊铺 |
| TLS | Tactical Logistics Systems | 战术后勤系统 |
| TLS | Tekniska Litteratursällskapet (Swedish Society for Technical Documentation) | Tekniska Litteratursällskapet (Swedish Society for Technical Documentation) |
| TLS | Triple Link Suspension | 三连杆悬架 |
| TLS | Tactical Landing Strip | 战术着陆带 |
| TLS | Trzy Literowy SkrÓt (Polish: three letter acronym) | Trzy Literowy SkrÓt (Polish: three letter acronym) |
| TLS | Transaction Level Security | 事务级别安全 |
| TLS | Third Level Support | 第三级支持 |
| TLS | Triple Lead Sheath | 三导联护套 |
| TLS | Transfer-Line Spill | 传输线溢出 |
| TLS | Transmission-Line Stamp | 输电线路印记 |
| TLS | Typed Letter Signed (autographs) | 签名的打印信函（签名） |