| VLS | Vehicle Location System | 车辆定位系统 |
| VLS | Vehicle Location Service | 车辆定位服务 |
| VLS | Village Voice Literary Supplement | 乡村之声文学副刊 |
| VLS | Virtual Learning System | 虚拟学习系统 |
| VLS | Valley Library Consortium (Saginaw, Michigan) | 山谷图书馆联盟（密歇根州萨吉诺） |
| VLS | Volunteer Life Saving Corps | 志愿救生队 |
| VLS | Vehicle Lease and Service Limited (UK) | 车辆租赁和服务有限公司（英国） |
| VLS | Vertical Lay System (subsea production wells) | 垂直铺设系统（海底生产井） |
| VLS | Vinyl Single | 乙烯基单体 |
| VLS | Vandenberg Launch and Landing Site | 范登堡发射和着陆场 |
| VLS | Variable Low Speed | 可变低速 |
| VLS | Virtual Learning Space | 虚拟学习空间 |
| VLS | Virtual Library System | 虚拟图书馆系统 |
| VLS | Vapor-Liquid-Solid | 蒸汽-液体-固体 |
| VLS | Vertical Launch System | 垂直发射系统 |
| VLS | VÉlos en Libre Service (French: free bicycling service) | VÉlos en Libre Service (French: free bicycling service) |
| VLS | Very Long Shot | 非常长的镜头 |
| VLS | VideoLAN Server | VideoLAN服务器 |
| VLS | Veiculo Lancador De Satelites | Veiclo Lancador De Satelites公司 |
| VLS | Villanova University Law School | 维拉诺瓦大学法学院 |
| VLS | Valesdir, Vanuatu | 瓦列斯迪尔，瓦努阿图 |
| VLS | VisionPlus Loyalty system | VisionPlus忠诚度系统 |
| VLS | Volume Loadability Speed | 体积负载能力速度 |
| VLS | Virgin Lip Syndrome | 处女唇综合征 |