| NLO | Nonlinear Optics | 非线性光学 |
| NLO | Nobody Likes Onions (website) | 没人喜欢洋葱（网站） |
| NLO | National Liaison Officer | 国家联络官 |
| NLO | No Load Operation (computer power supplies) | 空载运行（计算机电源） |
| NLO | NETg Learning Object | NETg学习对象 |
| NLO | No Lazy Offense | 不要偷懒 |
| NLO | Next-to-Leading Order | 前导订单旁边 |
| NLO | Nasolacrimal Obstruction | 鼻泪管阻塞 |
| NLO | National Labor Office | 国家劳工局 |
| NLO | Naval Liaison Officer | 海军联络官 |
| NLO | National Lead of Ohio Inc. | 俄亥俄州国家领导公司。 |
| NLO | New Little Odessa (gaming, Half-Life 2) | 新小敖德萨（游戏，半条命2） |
| NLO | Nepoznati Leteci Objekt (Croatian: unidentified flying object) | Nepoznati Leteci Objekt（克罗地亚语：不明飞行物体） |
| NLO | Nasolacrimal Occlusion | 鼻泪管阻塞 |
| NLO | Non-Linear Optic | 非线性光学 |
| NLO | NEW Leadership Oklahoma | 俄克拉荷马州新领导力 |
| NLO | Northern Lights Orchestra | 北极光管弦乐团 |
| NLO | Natural Language Query | 自然语言查询 |
| NLO | Nonlinear Optics: Materials, Phenomena and Devices | 非线性光学：材料、现象和器件 |
| NLO | Next-to-Leading-Order | 前导订单旁边 |