| OST | Original Sound Track | 影视原声大碟 |
| OST | Office of Science and Technology | （美国）科学技术局 |
| OST | Open Source Teaching | 开源教学 |
| OST | Objective, Strategy, Tactics (business/marketing) | 目标、策略、战术(商业/销售) |
| OST | Observatoire des Sciences et Techniques (French: science and technology observatory) | 科学和技术观测站（法语：科学和技术天文台） |
| OST | Optical Standards Tester (Carcadiant Systems) | 光学标准测试仪（致癌物系统） |
| OST | Old Spanish Trail | 西班牙古道 |
| OST | Office of the Secretary of Transportation | 交通部长办公室 |
| OST | Outer Space Treaty | 外层空间条约 |
| OST | Operational Suitability Test | 操作适用性测试 |
| OST | On-Screen Takeoff (construction planning) | 屏幕起飞（施工规划） |
| OST | Odonata Survey of Texas | 德克萨斯州Odonata调查 |
| OST | On Second Thought | 第二次思考 |
| OST | Over-Stress Test | 过度压力测试 |
| OST | Optical Switch Technology | 光交换技术 |
| OST | Out of School Time (after-school care programs) | 校外时间（课后护理计划） |
| OST | Operational Requirements Document Support Team | 作战需求文档支持团队 |
| OST | Order & Shipping Time | 秩序和运输时间 |
| OST | OPTEC System Team | OPTEC系统团队 |
| OST | Official Sound Track | 官方音轨 |
| OST | Operation Stress Test | 操作压力测试 |
| OST | Osmotic Shock Therapy | 渗透性休克疗法 |
| OST | Original Soundtrack The | 原声带The |
| OST | Orcish Stone Thrower | 兽人石头投掷者 |
| OST | Observed Sectional Trial | 观察截面试验 |
| OST | Austria Fund, Inc. | 奥地利基金公司。 |
| OST | Ocean Surface Temperature | 海洋表面温度 |
| OST | On Site Training | 现场培训 |
| OST | Oblate School of Theology | Oblate神学学院 |
| OST | Open Storage (software) | 开放存储（软件） |
| OST | Orthogonal Space-Time (coding scheme) | 正交空时（编码方案） |
| OST | Open Skies Treaty | 开放天空条约 |
| OST | Offline Store | 脱机存储 |
| OST | Operational Sea Training | 海上作战训练 |
| OST | Oscillating Sand Abrasion Test | 振动砂磨损试验 |
| OST | Oral Specimen Test | 口腔样本测试 |
| OST | Originating Station Treatment | 始发站处理 |
| OST | Observational Sequence Table | 观测序列表 |
| OST | Ormat Solar Turbine | Ormat太阳能涡轮机 |
| OST | oxytocin sensitivity test | 催产素敏感性试验 |
| OST | Office of the Special Trustee for American Indians | 美洲印第安人特别受托人办公室 |
| OST | object sorting test | 物体分类试验 |
| OST | Operations Support Team | 作战支援小队 |
| OST | Office of Secure Transportation (US Department of Energy) | 安全运输办公室（美国能源部） |
| OST | Official Soundtrack | 官方原声音乐大碟 |
| OST | Optimal Solutions and Technologies (Washington, DC) | 最佳解决方案和技术（华盛顿特区） |
| OST | Order Ship Time | 补充物资的运送时间 |
| OST | Office of Security Transition | 安全过渡办公室 |